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anti caspase 3 rabbit polyclonal ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti caspase 3 rabbit polyclonal ab
    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
    Anti Caspase 3 Rabbit Polyclonal Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+caspase+3/bio_rxiv__64898__2026__04__01__715697-96-1-5
    Average 86 stars, based on 1 article reviews
    anti caspase 3 rabbit polyclonal ab - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Quinolinic acid phosphoribosyl transferase moonlights as an apoptosis regulator to empower lung cancer progression"

    Article Title: Quinolinic acid phosphoribosyl transferase moonlights as an apoptosis regulator to empower lung cancer progression

    Journal: bioRxiv

    doi: 10.64898/2026.04.01.715697

    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) Caspase-3/7 activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
    Figure Legend Snippet: (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) Caspase-3/7 activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.

    Techniques Used: Staining, Knockdown, Cell Culture, Western Blot, Co-Immunoprecipitation Assay, Activity Assay, Purification, Immunoprecipitation

    In NSCLC cells as diagrammed in de novo NAD + biosynthesis is inactive, and NAD + pools are fueled by the salvage pathways and by the Preiss-Handler pathway. Instead, QPRT functions as an apoptosis inhibitor by interacting with caspase 3 and preventing its activation thereby conferring NSCLC resistance to apoptosis and enabling them to thrive through the stresses of tumor progression (2) .
    Figure Legend Snippet: In NSCLC cells as diagrammed in de novo NAD + biosynthesis is inactive, and NAD + pools are fueled by the salvage pathways and by the Preiss-Handler pathway. Instead, QPRT functions as an apoptosis inhibitor by interacting with caspase 3 and preventing its activation thereby conferring NSCLC resistance to apoptosis and enabling them to thrive through the stresses of tumor progression (2) .

    Techniques Used: Activation Assay

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    Chromatin Immunoprecipitation:

    Article Title: Orientia tsutsugamushi modulates p53, the cell cycle, and genotoxicity to maintain its intracellular niche
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    Flow Cytometry:

    Article Title: Orientia tsutsugamushi modulates p53, the cell cycle, and genotoxicity to maintain its intracellular niche
    Article Snippet: .. Blinding was not relevant to our study as there was no potential for interpretation bias. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Mouse monoclonal anti-p53 (Santa Cruz [sc-98]; 1:200), Rabbit monoclonal anti-p21 (Cell Signaling [#2947]; 1:1,000) Mouse monoclonal anti-HDM2 (Invitrogen [#33-7100]; 1:500) Mouse monoclonal anti-Cyclin E (Santa Cruz [sc-377100]; 1:500) Mouse monoclonal anti-Cyclin A (Santa Cruz [sc-271682]; 1:250) Mouse monoclonal anti-Cyclin D3 (Cell Signaling [#2936]; 1:1,000) Rabbit monoclonal anti-Cyclin D1 (Cell Signaling [#55506]; 1:1,000) Rabbit polyclonal anti-Cyclin B1 (Cell Signaling [#4138]; 1:1,000) Rabbit monoclonal anti-TCF11/NRF1 (Cell Signaling [#8052]; 1:1,000) Rabbit polyclonal anti-GFP (Invitrogen [A11122]; 1:1000) Rabbit monoclonal anti-GMNN (Cell Signaling [#52508]; 1:1,000) Rabbit polyclonal anti-cdc25a (Cell Signaling [#3652]; 1:1,000) Rabbit polyclonal anti-phospho-Histone H2A.X (yH2AX) (Cell Signaling [#2577]; 1:1,1000) Rabbit polyclonal anti-Caspase 7 (Cell Signaling [#9492]; 1:1,000) Rabbit polyclonal anti-Caspase 3 (Cell Signaling [#9662]; 1:1,000) Rabbit monoclonal anti-PARP1 (Invitrogen [MA5-15031]; 1:1,000) Rabbit polyclonal anti-PKR (Cell signaling [#3072; 1:1,000) Rabbit monoclonal anti-p-PKR (T446) (Abcam [ab32036]; 1:1,000) Rabbit polyclonal anti-TSA56 (Beyer et al., 2015) N/A (1:1,000) Mouse monoclonal anti-GAPDH (Santa Cruz [sc-365062]; 1:750) Horse anti-mouse, HRP (Cell Signaling Technology [#7076]; 1:10,000) Horse anti-rabbit, HRP (Cell Signaling Technology [#7074]; 1:10,000) Goat anti-rabbit IgG, Alexa Fluor 488 Invitrogen Cat#A-11034 TSA56 (an abundantly expressed O. tsutsugamushi outer membrane protein) antibody was validated by the absence of bands in uninfected samples and the visualized molecular weight band matching that of its predicted molecular weight. ..

    Magnetic Resonance Imaging:

    Article Title: Orientia tsutsugamushi modulates p53, the cell cycle, and genotoxicity to maintain its intracellular niche
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    Biomarker Discovery:

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    Membrane:

    Article Title: Orientia tsutsugamushi modulates p53, the cell cycle, and genotoxicity to maintain its intracellular niche
    Article Snippet: .. Blinding was not relevant to our study as there was no potential for interpretation bias. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Mouse monoclonal anti-p53 (Santa Cruz [sc-98]; 1:200), Rabbit monoclonal anti-p21 (Cell Signaling [#2947]; 1:1,000) Mouse monoclonal anti-HDM2 (Invitrogen [#33-7100]; 1:500) Mouse monoclonal anti-Cyclin E (Santa Cruz [sc-377100]; 1:500) Mouse monoclonal anti-Cyclin A (Santa Cruz [sc-271682]; 1:250) Mouse monoclonal anti-Cyclin D3 (Cell Signaling [#2936]; 1:1,000) Rabbit monoclonal anti-Cyclin D1 (Cell Signaling [#55506]; 1:1,000) Rabbit polyclonal anti-Cyclin B1 (Cell Signaling [#4138]; 1:1,000) Rabbit monoclonal anti-TCF11/NRF1 (Cell Signaling [#8052]; 1:1,000) Rabbit polyclonal anti-GFP (Invitrogen [A11122]; 1:1000) Rabbit monoclonal anti-GMNN (Cell Signaling [#52508]; 1:1,000) Rabbit polyclonal anti-cdc25a (Cell Signaling [#3652]; 1:1,000) Rabbit polyclonal anti-phospho-Histone H2A.X (yH2AX) (Cell Signaling [#2577]; 1:1,1000) Rabbit polyclonal anti-Caspase 7 (Cell Signaling [#9492]; 1:1,000) Rabbit polyclonal anti-Caspase 3 (Cell Signaling [#9662]; 1:1,000) Rabbit monoclonal anti-PARP1 (Invitrogen [MA5-15031]; 1:1,000) Rabbit polyclonal anti-PKR (Cell signaling [#3072; 1:1,000) Rabbit monoclonal anti-p-PKR (T446) (Abcam [ab32036]; 1:1,000) Rabbit polyclonal anti-TSA56 (Beyer et al., 2015) N/A (1:1,000) Mouse monoclonal anti-GAPDH (Santa Cruz [sc-365062]; 1:750) Horse anti-mouse, HRP (Cell Signaling Technology [#7076]; 1:10,000) Horse anti-rabbit, HRP (Cell Signaling Technology [#7074]; 1:10,000) Goat anti-rabbit IgG, Alexa Fluor 488 Invitrogen Cat#A-11034 TSA56 (an abundantly expressed O. tsutsugamushi outer membrane protein) antibody was validated by the absence of bands in uninfected samples and the visualized molecular weight band matching that of its predicted molecular weight. ..

    Molecular Weight:

    Article Title: Orientia tsutsugamushi modulates p53, the cell cycle, and genotoxicity to maintain its intracellular niche
    Article Snippet: .. Blinding was not relevant to our study as there was no potential for interpretation bias. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Mouse monoclonal anti-p53 (Santa Cruz [sc-98]; 1:200), Rabbit monoclonal anti-p21 (Cell Signaling [#2947]; 1:1,000) Mouse monoclonal anti-HDM2 (Invitrogen [#33-7100]; 1:500) Mouse monoclonal anti-Cyclin E (Santa Cruz [sc-377100]; 1:500) Mouse monoclonal anti-Cyclin A (Santa Cruz [sc-271682]; 1:250) Mouse monoclonal anti-Cyclin D3 (Cell Signaling [#2936]; 1:1,000) Rabbit monoclonal anti-Cyclin D1 (Cell Signaling [#55506]; 1:1,000) Rabbit polyclonal anti-Cyclin B1 (Cell Signaling [#4138]; 1:1,000) Rabbit monoclonal anti-TCF11/NRF1 (Cell Signaling [#8052]; 1:1,000) Rabbit polyclonal anti-GFP (Invitrogen [A11122]; 1:1000) Rabbit monoclonal anti-GMNN (Cell Signaling [#52508]; 1:1,000) Rabbit polyclonal anti-cdc25a (Cell Signaling [#3652]; 1:1,000) Rabbit polyclonal anti-phospho-Histone H2A.X (yH2AX) (Cell Signaling [#2577]; 1:1,1000) Rabbit polyclonal anti-Caspase 7 (Cell Signaling [#9492]; 1:1,000) Rabbit polyclonal anti-Caspase 3 (Cell Signaling [#9662]; 1:1,000) Rabbit monoclonal anti-PARP1 (Invitrogen [MA5-15031]; 1:1,000) Rabbit polyclonal anti-PKR (Cell signaling [#3072; 1:1,000) Rabbit monoclonal anti-p-PKR (T446) (Abcam [ab32036]; 1:1,000) Rabbit polyclonal anti-TSA56 (Beyer et al., 2015) N/A (1:1,000) Mouse monoclonal anti-GAPDH (Santa Cruz [sc-365062]; 1:750) Horse anti-mouse, HRP (Cell Signaling Technology [#7076]; 1:10,000) Horse anti-rabbit, HRP (Cell Signaling Technology [#7074]; 1:10,000) Goat anti-rabbit IgG, Alexa Fluor 488 Invitrogen Cat#A-11034 TSA56 (an abundantly expressed O. tsutsugamushi outer membrane protein) antibody was validated by the absence of bands in uninfected samples and the visualized molecular weight band matching that of its predicted molecular weight. ..

    Cell-Signaling:

    Article Title: Restraint of inflammasome-driven cytokine responses through the mRNA stability protein TTP
    Article Snippet: Rabbit monoclonal anti-GSDMD , Abcam , Cat# ab209845; RRID:AB_2783550. .. Rabbit polyclonal anti-caspase-3 , Cell Signaling Technology , Cat# 9662; RRID:AB_331439. .. Rabbit polyclonal anti-phospho-p38 (Thr180/Tyr182) , Cell Signaling Technology , Cat# 9211; RRID:AB_331641.



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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
    Cleaved Caspase 3 Asp175 Rabbit Polyclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
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    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) <t>Caspase-3/7</t> activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.
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    Image Search Results


    (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) Caspase-3/7 activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.

    Journal: bioRxiv

    Article Title: Quinolinic acid phosphoribosyl transferase moonlights as an apoptosis regulator to empower lung cancer progression

    doi: 10.64898/2026.04.01.715697

    Figure Lengend Snippet: (A–B) Annexin V, CF450 staining (0.25 µg/ml) in NSCLC cells with QPRT knockdown cultured in 2D (A) and 3D conditions with representative spheroid images (B) (n = 4). (C) Quantification of cell viability using PI staining for PC9, H2009 and H2030, and SYTOX Green for H1581 cells with QPRT silencing upon Z-VAD-FMK (50 µM for PC9, H2009 and H2030, and 100 µM for H1581) treatment (n = 4). (D) Immunoblots for co-IP experiments of V5-QPRT and its interaction with pro-caspase 3 in PC9, H2009 and H2030 cells. (E) Caspase-3/7 activity level in NSCLC cells with QPRT knockdown (n = 4). (F) Ribbon representation of QPRT (PDB: 5AYY) in complex with quinolinic acid with active site residues shown as sticks. Residues are colored according to ConSurf conservation scores . (G) Measurement of QPRT enzymatic activity over time of wild-type and catalytically inactive QPRT mutants purified form bacterial cells (n = 3). (H) Immunoblots for caspase-3 co-immunoprecipitated with catalytically inactive QPRT K139A versus wild-type QPRT. Data are represented as the mean ± SEM with statistical significance measured by one-way ANOVA followed by Dunnett’s post hoc test for panels A, B, and E, and followed by Tukey’s post hoc test for panel E. For the graph in panel G statistical significance was measured by two-way repeated measures ANOVA followed by Dunnett’s post hoc test and p value for the last time point is shown on the graphs. Scale bars in B indicate 400 µm.

    Article Snippet: For anti-Caspase-3 rabbit polyclonal Ab (Cell Signaling Technology, 9662S) 5% BSA in TBST was used to block the membranes instead of milk.

    Techniques: Staining, Knockdown, Cell Culture, Western Blot, Co-Immunoprecipitation Assay, Activity Assay, Purification, Immunoprecipitation

    In NSCLC cells as diagrammed in de novo NAD + biosynthesis is inactive, and NAD + pools are fueled by the salvage pathways and by the Preiss-Handler pathway. Instead, QPRT functions as an apoptosis inhibitor by interacting with caspase 3 and preventing its activation thereby conferring NSCLC resistance to apoptosis and enabling them to thrive through the stresses of tumor progression (2) .

    Journal: bioRxiv

    Article Title: Quinolinic acid phosphoribosyl transferase moonlights as an apoptosis regulator to empower lung cancer progression

    doi: 10.64898/2026.04.01.715697

    Figure Lengend Snippet: In NSCLC cells as diagrammed in de novo NAD + biosynthesis is inactive, and NAD + pools are fueled by the salvage pathways and by the Preiss-Handler pathway. Instead, QPRT functions as an apoptosis inhibitor by interacting with caspase 3 and preventing its activation thereby conferring NSCLC resistance to apoptosis and enabling them to thrive through the stresses of tumor progression (2) .

    Article Snippet: For anti-Caspase-3 rabbit polyclonal Ab (Cell Signaling Technology, 9662S) 5% BSA in TBST was used to block the membranes instead of milk.

    Techniques: Activation Assay